Objectives |
to characterize the proteome from Arabidopsis ecotypes selected for contrasted responses to environmental conditions |
Strategy |
screening of a collection of 76 accessions for changes in root system architecture under phosphate starvation (Chevalier et al., 2003; pdf) |
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root proteome analysis by two-dimensional gel electrophoresis and MALDI-TOF MS, hierarchical clustering of data (Chevalier et al., 2004; pdf) |
Results |
annotated 2D maps of root proteins from 8 Arabidopsis ecotypes |
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- 2 very close accessions (Col 0, Col 4) showing the classical double response to phosphate starvation (decrease in both lenght of primary root and number of lateral roots) |
 |  | Col 0 | Col 4 |
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- 3 widely-used accessions (Cvi 0, Ler 1, Ws 1) showing also the classical double response |
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- and 3 accessions (Be 0, Ll 0, Rld 1) showing only one of the two responses |
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classification of proteomes according to protein maps and functions |
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