GENSCAN 1.0 Date run: 6-Jul-101 Time: 10:17:19 Sequence locusA : 5040 bp : 36.47% C+G : Isochore 1 ( 0 - 43 C+G%) Parameter matrix: HumanIso.smat Predicted genes/exons: Gn.Ex Type S .Begin ...End .Len Fr Ph I/Ac Do/T CodRg P.... Tscr.. ----- ---- - ------ ------ ---- -- -- ---- ---- ----- ----- ------ 1.01 Init + 1629 2617 989 2 2 62 76 802 0.756 69.20 1.02 Term + 2696 3980 1285 2 1 91 54 1248 0.998 111.76 1.03 PlyA + 4116 4121 6 1.05Click here to view a PDF image of the predicted gene(s)
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Predicted peptide sequence(s): >locusA|GENSCAN_predicted_peptide_1|757_aa MFNHPWPNLTLIYFFFIVVLPFQSLSQFDSPQNIETFFPISSLSPVPPPLLPPSSNPSPP SNNSSSSDKKTITKAVLITAASTLLVAGVFFFCLQRCIIARRRRDRVGPVRVENTLPPYP PPPMTSAAVTTTTLAREGFTRFGGVKGLILDENGLDVLYWRKLQSQRERSGSFRKQIVTG EEEDEKEVIYYKNKKKTEPVTEIPLLRGRSSTSHSVIHNEDHQPPPQVKQSEPTPPPPPP SIAVKQSAPTPSPPPPIKKGSSPSPPPPPPVKKVGALSSSASKPPPAPVRGASGGETSKQ VKLKPLHWDKVNPDSDHSMVWDKIDRGSFSFDGDLMEALFGYVAVGKKSPEQGDEKNPKS TQIFILDPRKSQNTAIVLKSLGMTREELVESLIEGNDFVPDTLERLARIAPTKEEQSAIL EFDGDTAKLADAETFLFHLLKSVPTAFTRLNAFLFRANYYPEMAHHSKCLQTLDLACKEL RSRGLFVKLLEAILKAGNRMNAGTARGNAQAFNLTALLKLSDVKSVDGKTSLLNFVVEEV VRSEGKRCVMNRRSHSLTRSGSSNYNGGNSSLQVMSKEEQEKEYLKLGLPVVGGLSSEFS NVKKAACVDYETVVATCSALAVRAKDAKTVIGECEDGEGGRFVKTMMTFLDSVEEEVKIA KGEERKVMELVKRTTDYYQAGAVTKGKNPLHLFVIVRDFLAMVDKVCLDIMRNMQRRKVG SPISPSSQRNAVKFPVLPPNFMSDRAWSDSGGSDSDM Explanation Gn.Ex : gene number, exon number (for reference) Type : Init = Initial exon (ATG to 5' splice site) Intr = Internal exon (3' splice site to 5' splice site) Term = Terminal exon (3' splice site to stop codon) Sngl = Single-exon gene (ATG to stop) Prom = Promoter (TATA box / initation site) PlyA = poly-A signal (consensus: AATAAA) S : DNA strand (+ = input strand; - = opposite strand) Begin : beginning of exon or signal (numbered on input strand) End : end point of exon or signal (numbered on input strand) Len : length of exon or signal (bp) Fr : reading frame (a forward strand codon ending at x has frame x mod 3) Ph : net phase of exon (exon length modulo 3) I/Ac : initiation signal or 3' splice site score (tenth bit units) Do/T : 5' splice site or termination signal score (tenth bit units) CodRg : coding region score (tenth bit units) P : probability of exon (sum over all parses containing exon) Tscr : exon score (depends on length, I/Ac, Do/T and CodRg scores) Comments The SCORE of a predicted feature (e.g., exon or splice site) is a log-odds measure of the quality of the feature based on local sequence properties. For example, a predicted 5' splice site with score > 100 is strong; 50-100 is moderate; 0-50 is weak; and below 0 is poor (more than likely not a real donor site). The PROBABILITY of a predicted exon is the estimated probability under GENSCAN's model of genomic sequence structure that the exon is correct. This probability depends in general on global as well as local sequence properties, e.g., it depends on how well the exon fits with neighboring exons. It has been shown that predicted exons with higher probabilities are more likely to be correct than those with lower probabilities.