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CLUSTAL W (1.81) Multiple Sequence Alignments Sequence format is Pearson Sequence 1: gi|6503010|gb|AAF14548.1| 1051 aa Sequence 2: Bprotein 426 aa Sequence 3: gi|6691125 417 aa Sequence 4: gi|6041849 373 aa Sequence 5: gi|6225268 443 aa Sequence 6: gi|544344 406 aa Sequence 7: gi|2281090 416 aa Sequence 8: gi|1061334 437 aa Sequence 9: gi|5080823 421 aa Start of Pairwise alignments Aligning... Sequences (1:2) Aligned. Score: 35.2113 Sequences (1:3) Aligned. Score: 71.7026 Sequences (1:4) Aligned. Score: 42.0912 Sequences (1:5) Aligned. Score: 20.0903 Sequences (1:6) Aligned. Score: 17.9803 Sequences (1:7) Aligned. Score: 60.8173 Sequences (1:8) Aligned. Score: 17.3913 Sequences (1:9) Aligned. Score: 30.8789 Sequences (2:2) Aligned. Score: 100 Sequences (2:3) Aligned. Score: 34.0528 Sequences (2:4) Aligned. Score: 24.933 Sequences (2:5) Aligned. Score: 19.0141 Sequences (2:6) Aligned. Score: 16.5025 Sequences (2:7) Aligned. Score: 34.6154 Sequences (2:8) Aligned. Score: 15.493 Sequences (2:9) Aligned. Score: 56.057 Sequences (3:2) Aligned. Score: 34.0528 Sequences (3:3) Aligned. Score: 100 Sequences (3:4) Aligned. Score: 39.6783 Sequences (3:5) Aligned. Score: 22.3022 Sequences (3:6) Aligned. Score: 15.7635 Sequences (3:7) Aligned. Score: 62.2596 Sequences (3:8) Aligned. Score: 16.307 Sequences (3:9) Aligned. Score: 29.4964 Sequences (4:2) Aligned. Score: 24.933 Sequences (4:3) Aligned. Score: 39.6783 Sequences (4:4) Aligned. Score: 100 Sequences (4:5) Aligned. Score: 18.4987 Sequences (4:6) Aligned. Score: 13.6729 Sequences (4:7) Aligned. Score: 32.4397 Sequences (4:8) Aligned. Score: 15.2815 Sequences (4:9) Aligned. Score: 21.1796 Sequences (5:2) Aligned. Score: 19.0141 Sequences (5:3) Aligned. Score: 22.3022 Sequences (5:4) Aligned. Score: 18.4987 Sequences (5:5) Aligned. Score: 100 Sequences (5:6) Aligned. Score: 19.9507 Sequences (5:7) Aligned. Score: 19.2308 Sequences (5:8) Aligned. Score: 18.7643 Sequences (5:9) Aligned. Score: 19.4774 Sequences (6:2) Aligned. Score: 16.5025 Sequences (6:3) Aligned. Score: 15.7635 Sequences (6:4) Aligned. Score: 13.6729 Sequences (6:5) Aligned. Score: 19.9507 Sequences (6:6) Aligned. Score: 100 Sequences (6:7) Aligned. Score: 16.0099 Sequences (6:8) Aligned. Score: 33.9901 Sequences (6:9) Aligned. Score: 15.0246 Sequences (7:2) Aligned. Score: 34.6154 Sequences (7:3) Aligned. Score: 62.2596 Sequences (7:4) Aligned. Score: 32.4397 Sequences (7:5) Aligned. Score: 19.2308 Sequences (7:6) Aligned. Score: 16.0099 Sequences (7:7) Aligned. Score: 100 Sequences (7:8) Aligned. Score: 16.5865 Sequences (7:9) Aligned. Score: 32.2115 Sequences (8:2) Aligned. Score: 15.493 Sequences (8:3) Aligned. Score: 16.5468 Sequences (8:4) Aligned. Score: 15.2815 Sequences (8:5) Aligned. Score: 18.7643 Sequences (8:6) Aligned. Score: 32.266 Sequences (8:7) Aligned. Score: 16.5865 Sequences (8:8) Aligned. Score: 100 Sequences (8:9) Aligned. Score: 15.2019 Sequences (9:2) Aligned. Score: 56.057 Sequences (9:3) Aligned. Score: 29.4964 Sequences (9:4) Aligned. Score: 21.1796 Sequences (9:5) Aligned. Score: 19.4774 Sequences (9:6) Aligned. Score: 15.0246 Sequences (9:7) Aligned. Score: 32.2115 Sequences (9:8) Aligned. Score: 15.2019 Sequences (9:9) Aligned. Score: 100 Guide tree file created: [clustal.dnd] Start of Multiple Alignment There are 8 groups Aligning... Group 1: Sequences: 2 Score:7195 Group 2: Sequences: 2 Score:7718 Group 3: Sequences: 3 Score:7204 Group 4: Sequences: 4 Score:5746 Group 5: Sequences: 6 Score:4332 Group 6: Delayed Group 7: Sequences: 2 Score:4728 Group 8: Sequences: 8 Score:3089 Sequence:5 Score:3266 Alignment Score 21682 CLUSTAL-Alignment file created [clustal.aln]

Clustal output

CLUSTAL W (1.81) multiple sequence alignment Bprotein -------------------------------------------------- gi|5080823 -------------------------------------------------- gi|6503010|gb|AAF14548.1| MLFFLFFFYLLLSSSSDLVFADRRVLHEPFFPIDSPPPSPPSPPPLPKLP gi|6691125 -------------------------------------------------- gi|2281090 -------------------------------------------------- gi|6041849 -------------------------------------------------- gi|544344 -------------------------------------------------- gi|1061334 -------------------------------------------------- gi|6225268 -------------------------------------------------- Bprotein -------------------------------------------------- gi|5080823 -------------------------------------------------- gi|6503010|gb|AAF14548.1| FSSTTPPSSSDPNASPFFPLYPSSPPPPSPASFASFPANISSLIVPHATK gi|6691125 -------------------------------------------------- gi|2281090 -------------------------------------------------- gi|6041849 -------------------------------------------------- gi|544344 -------------------------------------------------- gi|1061334 -------------------------------------------------- gi|6225268 -------------------------------------------------- Bprotein -------------------------------------------------- gi|5080823 -------------------------------------------------- gi|6503010|gb|AAF14548.1| SPPNSKKLLIVAISAVSSAALVALLIALLYWRRSKRNQDLNFSDDSKTYT gi|6691125 -------------------------------------------------- gi|2281090 -------------------------------------------------- gi|6041849 -------------------------------------------------- gi|544344 -------------------------------------------------- gi|1061334 -------------------------------------------------- gi|6225268 -------------------------------------------------- Bprotein -------------------------------------------------- gi|5080823 -------------------------------------------------- gi|6503010|gb|AAF14548.1| TDSSRRVYPPPPATAPPTRRNAEARSKQRTTTSSTNNNSSEFLYLGTMVN gi|6691125 -------------------------------------------------- gi|2281090 -------------------------------------------------- gi|6041849 -------------------------------------------------- gi|544344 -------------------------------------------------- gi|1061334 -------------------------------------------------- gi|6225268 -------------------------------------------------- Bprotein -------------------------------------------------- gi|5080823 -------------------------------------------------- gi|6503010|gb|AAF14548.1| QRGIDEQSLSNNGSSSRKLESPDLQPLPPLMKRSFRLNPDVGSIGEEDEE gi|6691125 -------------------------------------------------- gi|2281090 -------------------------------------------------- gi|6041849 -------------------------------------------------- gi|544344 -------------------------------------------------- gi|1061334 -------------------------------------------------- gi|6225268 -------------------------------------------------- Bprotein -------------------------------------------------- gi|5080823 -------------------------------------------------- gi|6503010|gb|AAF14548.1| DEFYSPRGSQSGREPLNRVGLPGQNPRSVNNDTISCSSSSSGSPGRSTFI gi|6691125 -------------------------------------------------- gi|2281090 -------------------------------------------------- gi|6041849 -------------------------------------------------- gi|544344 -------------------------------------------------- gi|1061334 -------------------------------------------------- gi|6225268 -------------------------------------------------- Bprotein -------------------------------------------------- gi|5080823 -------------------------------------------------- gi|6503010|gb|AAF14548.1| SISPSMSPKRSEPKPPVISTPEPAELTDYRFVRSPSLSLASLSSGLKNSD gi|6691125 -------------------------------------------------- gi|2281090 -------------------------------------------------- gi|6041849 -------------------------------------------------- gi|544344 -------------------------------------------------- gi|1061334 -------------------------------------------------- gi|6225268 -------------------------------------------------- Bprotein -------------------------------------------------- gi|5080823 -------------------------------------------------- gi|6503010|gb|AAF14548.1| EVGLNQIFRSPTVTSLTTSPENNKKENSPLSSTSTSPERRPNDTPEAYLR gi|6691125 -------------------------------------------------- gi|2281090 -------------------------------------------------- gi|6041849 -------------------------------------------------- gi|544344 -------------------------------------------------- gi|1061334 -------------------------------------------------- gi|6225268 -------------------------------------------------- Bprotein -------------------------------------------------- gi|5080823 -------------------------------------------------- gi|6503010|gb|AAF14548.1| SPSHSSASTSPYRCFQKSPEVLPAFMSNLRQGLQSQLLSSPSNSHGGQGF gi|6691125 -------------------------------------------------- gi|2281090 -------------------------------------------------- gi|6041849 -------------------------------------------------- gi|544344 -------------------------------------------------- gi|1061334 -------------------------------------------------- gi|6225268 -------------------------------------------------- Bprotein -------------------------------------------------- gi|5080823 -------------------------------------------------- gi|6503010|gb|AAF14548.1| LKQLDALRSRSPSSSSSSVCSSPEKASHKSPVTSPKLSSRNSQSLSSSPD gi|6691125 -------------------------------------------------- gi|2281090 -------------------------------------------------- gi|6041849 -------------------------------------------------- gi|544344 -------------------------------------------------- gi|1061334 -------------------------------------------------- gi|6225268 -------------------------------------------------- Bprotein -------------------------------------------------- gi|5080823 -------------------------------------------------- gi|6503010|gb|AAF14548.1| RDFSHSLDVSPRISNISPQILQSRVPPPPPPPPPLPLWGRRSQVTTKADT gi|6691125 -------------------------------------------------- gi|2281090 -------------------------------------------------- gi|6041849 -------------------------------------------------- gi|544344 -------------------------------------------------- gi|1061334 -------------------------------------------------- gi|6225268 -------------------------------------------------- Bprotein -------------------------------------SGGETSKQVKLKP gi|5080823 -------------------------------------GKTEDPTQPKLKP gi|6503010|gb|AAF14548.1| ISRPPSLTPPSHPFVIPSENLPVTSSPMETPETVCASEAAEETPKPKLKA gi|6691125 -------------------------------------EKSEEILKPKLKT gi|2281090 -------------------------------------EKKVETMKPKLKT gi|6041849 -------------------------------------EGTTDRPKPKLKP gi|544344 -------------------------------------RKPAIEPSCPMKP gi|1061334 -------------------------------------RKSAVNPPKPMRP gi|6225268 -------------------------------------PKKLYKPEVQLRR :: Bprotein LHWDKVNPDSDHS------------------------------------- gi|5080823 LHWDKMNPDASRS------------------------------------- gi|6503010|gb|AAF14548.1| LHWDKVRASSDRE------------------------------------- gi|6691125 LHWDKVRASSDCE------------------------------------- gi|2281090 LHWDKVRASSSRV------------------------------------- gi|6041849 LPWDKVRPSSRRT------------------------------------- gi|544344 LYWTRIQINDKSQDAAP--------------------------------- gi|1061334 LYWTRIVTSAPPAPRPPSVANSTDSTENSGSSPDEPPAANGADAPPTAPP gi|6225268 PNWSKLVAEDLSQD------------------------------------ * :: . Bprotein ---MVWDKIDRGSFSFDGDLMEALFGYVAVGKKSPEQGDEK-NPKS---- gi|5080823 ---MVWHKIDGGSFNFDGDLMEALFGYVARKPSESNSVPQN-QTVSNSVP gi|6503010|gb|AAF14548.1| ---MVWDHLRSSSFKLDEEMIETLFVAKSL-NNKPNQSQTT-PRCVLPSP gi|6691125 ---MVWDQLKSSSFKLNEEMIETLFVVK---NPTLNTSATA-KHFVVSSM gi|2281090 ---MVWDQIKSNSFQVNEEMIETLFKVN-------DPTSRT-RDGVVQSV gi|6041849 ---NTWDRLPYNSSNA-------------------NSKQRS-LSCDLPML gi|544344 ---TLWDSLEEPHIRDTSEFEYLFSKDTTQ---QKKKPLSE-AYEKKNKV gi|1061334 ATKEIWTEIEETPLDNIDEFTELFSR-------QAIAPVSK-PKELKVKR gi|6225268 ---CFWTKVKEDRFENNELFAKLTLTFSAQTKTKKDQEGGEEKKSVQKKK * : Bprotein -TQIFILDPRKSQNTAIVLKSLGMTREELVESLIEGN--DFVPDTLERLA gi|5080823 HNQTYILDPRKSQNKAIVLKSLGMTKEEIIDLLTEGH--DAESDTLEKLA gi|6503010|gb|AAF14548.1| NQENRVLDPKKAQNIAILLRALNVTIEEVCEALLEGNADTLGTELLESLL gi|6691125 SQENRVLDPKKSQNIAILLRVLNGTTEEICEAFLEGNAENIGTELLEILL gi|2281090 SQENRFLDPRKSHNIAILLRALNVTADEVCEALIEGNSDTLGPELLECLL gi|6041849 NQESKVLDPRKSQNVAVLLTTLKLTTNDVCQALRDGHYDALGVELLESLA gi|544344 KKIIKLLDGKRSQTVGILISSLHLEMKDIQQAIFTVDDSVVDLETLAALY gi|1061334 AKSIKVLDPERSRNVGIIWRSLHVPSSEIEHAIYHIDTSVVSLEALQHMS gi|6225268 VKELKVLDSKTAQNLSIFLGSFRMPYQEIKNVILEVNEAVLTESMIQNLI .** . ::. .:. : .:: . : . . : : Bprotein RIAPTKEEQSAILEFDGD----TAKLADAETFLFHLLKSVPTAFTRLNAF gi|5080823 GIAPTPEEQTEIIDFDGE----PMTLAYADSLLFHILKAVPSAFNRFNVM gi|6503010|gb|AAF14548.1| KMAPTKEEERKLKAYNDD---SPVKLGHAEKFLKAMLD-IPFAFKRVDAM gi|6691125 KMAPSKEEERKLKEYKDD---SPFKLGPAEKFLKAVLD-IPFAFKRIDAM gi|2281090 KMAPTKEEEDKLKELKDDDDGSPSKIGPAEKFLKALLN-IPFAFKRIDAM gi|6041849 RVAPSEEEEKKLISYSDD---SVIKLAPSERFLKELLN-VPFVFKRVDAL gi|544344 ENRAQEDELTKIRKYYETSKEEDLKLLDKPEQFLHELAQIPNFAERAQCI gi|1061334 NIQATEDELQRIKEAAGGDIP-----LDHPEQFLLDISLISMASERISCI gi|6225268 KQMPEPEQLKMLSELKDEY-----DDLAESEQFGVVMGTVPRLRPRLNAI . :: : : : :. * . : Bprotein LFRANYYPEMAHHSKCLQTLDLACKELRSRGLFVKLLEAILKAGNRMNAG gi|5080823 LFKINYGSEVAQQKGSLLTLESACNELRARGLFMKLLEAILKAGNRMNAG gi|6503010|gb|AAF14548.1| LYVANFESEVEYLKKSFETLEAACEELRNSRMFLKLLEAVLKTGNRMNVG gi|6691125 LYISNFDYEVDYLGNSFETLEAACEELRSSRMFLKLLEAVLKTGNRMNVG gi|2281090 LYIVKFESEIEYLNRSFDTLEAATGELKNTRMFLKLLEAVLKTGNRMNIG gi|6041849 LSVASFDSKVKHLKRSFSVIQAACEALRNSRMLLRLVGATLEAG------ gi|544344 IFRAVFSEGITSLHRKVEIVTRASKGLLHMKSVKDILALILAFGNYMNGG gi|1061334 VFQAEFEESVTLLFRKLETVSQLSQQLIESEDLKLVFSIILTLGNYMNGG gi|6225268 LFKLQFSEQVENIKPEIVSVTAACEELRKSESFSNLLEITLLVGNYMNAG : : : . : * . :. * * Bprotein -TARGNAQAFNLTALLKLSDVKSVDGKTSLLNFVVEEVVRSEGKRCVMNR gi|5080823 -TARGNAQAFNLTALRKLSDVKSVDAKTTLLHFVVEEVVRSEGKRAAMNK gi|6503010|gb|AAF14548.1| -TNRGDAHAFKLDTLLKLVDVKGADGKTTLLHFVVQEIIRAEGTRLS--- gi|6691125 -TNRGDAHAFKLDTLLKLVDVKGADGKTTLLHFVVQEIIKSEGARLS--- gi|2281090 -TNRGDAHAFKLDTLLKLVDIKGADGKTTLLHFVVQEIIKFEGARVPFT- gi|6041849 -MKSGNAHDFKLEALLGLVDIKSSDGRTSILDSVVQKITESEG------- gi|544344 NRTRGQADGYSLEILPKLKDVKSRDNGMNLVDYVVKYYLRYYDQE----- gi|1061334 NRQRGQADGFNLDILGKLKDVKSKESHTTLLHFIVRTYIAQRRKE----- gi|6225268 SRNAG-AFGFNISFLCKLRDTKSTDQKMTLLHFLAELCEN---------- * * :.: * * * *. : .::. :.. Bprotein RSHSLTRSGSSNYNGGNSSLQVMSKEEQEKEYLKLGLPVVGGLSSEFSNV gi|5080823 NMMS-----SDNGSGEN---ADMSREEQEIEFIKMGLPIIGGLSSEFTNV gi|6503010|gb|AAF14548.1| ---------------GNN------TQTDDIKCRKLGLQVVSSLCSELSNV gi|6691125 ---------------GGNQNHQQSTTNDDAKCKKLGLQVVSNISSELINV gi|2281090 ---------PSQSHIGDN-MAEQSAFQDDLELKKLGLQVVSGLSSQLINV gi|6041849 ---------------------------------IKGLQVVRNLSSVLNDA gi|544344 ---------------------------------AGTDKSV-FPLPEPQDF gi|1061334 ---------------------------------GVHPLEIRLPIPEPADV gi|6225268 -----------------------------------DYPDVLKFPDELAHV : . Bprotein KKAACVDYETVVATCSALAVRAKDAKTVIGE------CEDGEGGRFVKTM gi|5080823 KKAAGIDYDSFVATTLALGTRVKETKRLLDQ------SKGKEDGCLTK-L gi|6503010|gb|AAF14548.1| KKAAAMDSEVLSSYVSKLSQGIAKINEAIQ--VQSTITEESNSQRFSESM gi|6691125 KKSAAMDSEVLHNDVLKLSKGIQNIAEVVRS-IEAVGLEESSIKRFSESM gi|2281090 KKAAAMDSNSLINETAEIARGIAKVKEVIT-----ELKQETGVERFLESM gi|6041849 KKSAELDYGVVRMNVSKLYEEVQKISEVLRLCEETGHSEEHQWWKFRESV gi|544344 FLASQVKFEDLLKDLRKLKRQLEASEQQMKL--VCKESPREYLQPFKDKL gi|1061334 ERAAQMDFEEVQQQIFDLNKKFLGCKRTTAK--VLAASRPEIMEPFKSKM gi|6225268 EKASRVSAENLQKNLDQMKKQISDVERDVQN----FPAATDEKDKFVEKM :: :. . : : . : Bprotein MTFLDSVEEEVKIAKGEERKVMELVKRTTDYYQAGAV-----TKGKNPLH gi|5080823 RSFFESAEEELKVITEEQLRIMELVKKTTNYYQAGAL-----KE-RNLFQ gi|6503010|gb|AAF14548.1| KTFLKRAEEEIIRVQAQESVALSLVKEITEYFHGNSA-----KEEAHPFR gi|6691125 NRFMKVAEEKILRLQAQETLAMSLVKEITEYVHGDSA-----REEAHPFR gi|2281090 NSFLNKGEKEITELQSHGDNVMKMVKEVTEYFHGNS--------ETHPFR gi|6041849 TRFLETAAEEIKKIEREEGSTLFAVKKITEYFHVDPA-----KEEAQLLK gi|544344 EEFFKKAKKEHKMEESHLENAQKSFETTVGYFGMKPKTGEK---EVTPSY gi|1061334 EEFVEGADKSMAKLHQSLDECRDLFLETMRFYHFSPKACTLTLAQCTPDQ gi|6225268 TSFVKDAQEQYNKLRMMHSNMETLYKELGEYFLFDPK-------KLSVEE *.. :. : . Bprotein LFVIVRDFLAMVDKVCLDIMRNMQRRK----------------------- gi|5080823 LFVIIRDFLGMVDNACSEIARNQRKQQ----------------------- gi|6503010|gb|AAF14548.1| IFLVVRDFLGVVDRVCKEVGMINERTMVSSAHKFPVPVNPMMPQPLPGLV gi|6691125 IFMVVKDFLMILDCVCKEVGTINERTI----------------------- gi|2281090 IFAVVRDFLTILDQVCKEVGRVNERTV----------------------- gi|6041849 VFVIVRDFLKILEGVCKKMEVTSSLA------------------------ gi|544344 VFMVWFEFCSDFKTIWKRESKNISKER----------------------- gi|1061334 FFEYWTNFTNDFKDIWKKEITSLLNEL----------------------- gi|6225268 FFMDLHNFRNMFLQAVKENQKRRKTEEKMRRAKLAKEKAEKERLEKQQKR .* :* . Bprotein ------------------------------- gi|5080823 ------------------------------- gi|6503010|gb|AAF14548.1| GRRQSSSSSSSSSTSSSDEDEHNSISLVS-- gi|6691125 ------------------------------- gi|2281090 ------------------------------- gi|6041849 ------------------------------- gi|544344 ------------------------------- gi|1061334 ------------------------------- gi|6225268 EQLIDMNAEGDETGVMDSLLEALQSGAAFRR

RunClustalW--V1.3--18-Nov-1999/JackL