United Nations Educational, Scientific and Cultural Organization
Organisation des Nations Unies pour l' Education, la Science et la Culture

INTERNATIONAL CELL RESEARCH ORGANIZATION
ORGANISATION INTERNATIONALE DE RECHERCHE SUR LA CELLULE

Signalling to growth and cell division in Arabidopsis:

Program of the Workshop and Practical Course

Workshop

July 19, Thursday

Cell Cycle Control

8:45 Opening: L. Bogre
9:00 De Veylder, L. (Gent): Cell cycle control in Arabidopsis
9:30 J. Murray (Cambridge): G1 control
10:00 H. Nakagami (Tokyo): D-type cyclins - CDK-Cyclin partnerships
10:30 D. Dudits (Szeged): Plant CDKs

11: 00 BREAK

Spatial regulation of cell division

11:30 J. Doonan (Norwich): Cell divisions, meristem development
12:00 D. Francis (Cardiff): Cell cycle regulation in TBY-2 cells
12:30 L. Bogre (London): 4D cell cycle (GFP technologies)

13:00 LUNCH

Cell divisions, variations on the theme

15:00 H. Dickinson (Oxford): Endosperm development
15:30 A Bhatt (Oxford): Plant meiosis: the means to 1N

16:00 BREAK

Cell cycle kinetics

16:30 G. Beemster (Gent): Cell growth and cell division measurements
17:00 P. Laufs (Versailles): Reconstruction of cell divisions in shoot meristem
17:30 C. de la Torre (Madrid): Cell cycle dynamics, checkpoints (historical overview)

18:00 END - followed by the GET TOGETHER DINNER

July 20, Friday

Signalling kinases

9:00 Cs. Koncz (Cologne): SNF kinase
9:30 H. Hirt (Vienna): MAPK signalling
10:00 I. Meskiene (Vienna): MAPK signalling, negative regulation
10:30 K. Mockaitis (Orsey): MAPK signalling, going upstream

11:00 BREAK

Signalling

11:30 T Munnik (Amsterdam): Phospholipid signalling
12:00 M. Knight (Oxford): Ca2+ signalling
12:30 T. Romeis (Norwich/Cologne): CDPK

13:00 LUNCH

15:00 S. Peck (Norwich) Phosphoproteomics

Cytoskeleton

15:30 C. Lloyd (Norwich): Cortical microtubules and cell growth
16:00 P. Hussey (Durham): MAPs and signalling
16:30 P. Binarova (Prague): Microtubule nucleation

17:00 BREAK

Polarised growth

17:30 D. Bouchez (Versailles): PPB: Ton mutants
18:00 F. Cvrckova (Prague): Evolutionarily conserved themes in polarised growth – in silico approach
18:30 I. Moore (Oxford): Vesicle trafficking

19:00 END

July 21, Saturday

Reverse genetics

9:00 C. Koncz: T-DNA muatagenesis: Cologne perspective
9:30 D. Bouchez: T-DNA mutagenesis: Versailles perspective
10:00 J. Doonan/P. Laufs: Ethanol-inducible expression system
10:30 I. Moore: Inducible systems based on lacR

Practical course

July 23, Monday

9:00-9:30

Growth measurements on plant and cellular levels, measurements of cell cycle kinetics (G. Beemster)

Root marking, scanning
9:30-10:30 Observation - Time point 1
10:30-11:30 Observation - Time point 2
11:30-13:00 DIC imaging
LUNCH
14:00-16:00 Image analysis
16:00-16:45

Reconstruction of mitotic domains in Arabidopsis meristems from confocal sections (P. Laufs)
16:45-17:45

Bioinformatics (F. Cvrckova)

(to be continued in spare time throughout - PCs will be available for the rest of the course)
BREAK
19:00-?? Image analysis continued
Bioinformatics continued

July 24, Tuesday

9:00-10:30

Inducible expression systems (J. Doonan, P. Lauf)

Ethanol- and glucocorticoid- inducible expression in Arabidopsis: GUS staining, GFP observation
10:30-12:00 Ethanol-, glucocorticoid- and tetracycline-inducible expression in tobacco cell lines: observation of Cdc2::GFP, dominant negative Cdc2 mutant and cyclin::GFP - expressing cells
LUNCH
13:00-14:00

Transient gene expression in protoplasts (I. Meskiene)

Protoplasting
14:00-16:00

Synchronisation of Arabidopsis cells, BrdU labelling, flow cytometry (L. Bogre)
BREAK
17:00-20:00 Transient transformation of protoplasts using PEG

July 25, Wednesday

9:00-10:30 Evaluation of transformation efficiency - GFP observation.
Harvesting cells for kinase assays and GUS measurements.
10:30-12:00

Immunolabelling of cultured cells and seedlings (P. Binarova)
LUNCH
13:00-16:00 Immunolabelling continued
Bioinformatics (continued)
Optional: GUS and kinase assays on transiently transformed protoplasts
BREAK
17:00-20:00 Immunolabelling continued, mounting of slides, observation
Bioinformatics continued

July 26, Thursday

9:00-10:30 Observation and evaluation of immunolabelling
9:15-10:30 Evaluation of GUS and kinase assays, discussion of results
10:30-11:00 Bioinformatics: discussion of results
11:00-13:00

Finishing the unfinished + evaluation and discussion of course results 
END

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