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1. We used the PHYB::LUCIFERASE promoter::reporter gene construct to screen in vivo for disruption of the shade response in the 7 day old, light-grown Arabidopsis seedlings. The bio-luminescence ( shade responses) was tested shade treatment.
2. Map-based-cloning or positional cloning
A lots of PCR and Web database are needed
3. T-DNA insertional mutagenesis
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Update on 2006-2-23