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Technology

 

1.  We used the PHYB::LUCIFERASE promoter::reporter gene construct to screen in vivo for disruption of the shade response in the 7 day old, light-grown Arabidopsis seedlings. The bio-luminescence ( shade responses) was tested shade treatment.

 

 

2. Map-based-cloning or positional cloning

                 A lots of PCR and Web database  are needed

3. T-DNA insertional mutagenesis

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Update on  2006-2-23